F2168
Sigma
Monoclonal Anti-α-Tubulin−FITC antibody produced in mouse
clone DM1A, purified immunoglobulin, buffered aqueous solution
| Synonym: | Monoclonal Anti-α-Tubulin |
Description
| Immunogen | microtubules from chicken embryo brain. |
| Specificity | The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide |
Properties
| antibody form | purified immunoglobulin |
| clone | DM1A (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~50 kDa |
| species reactivity | yeast, human, chicken, rat, amphibian, fungi, bovine, mouse |
| storage condition | protect from light |
| application(s) | direct immunofluorescence: 1:50 using cultured BHK cells |
| isotype | IgG1 |
| Gene Information | human ... TUBA4A(7277) |
| rat ... Tuba1a(64158) | |
| mouse ... Tuba1a(22142) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Khodjakov, A., et al., Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis J. Cell Biol. 160, 671-683, (2003) Abstract |
| Saberi, A., et al., An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells J. Cell Biol. 178, 257-268, (2007) Abstract | |
| Rines, D.R., et al., A role for IkappaB kinase 2 in bipolar spindle assembly Proc. Natl. Acad. Sci. U. S. A. 104, 16940-16945, (2007) Abstract | |
| Blose, S.H., et al., 10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin. J. Cell Biol. 98, 847, (1984) |
